The techniques for establishing and maintaining invertebrate tissues and cells in culture remain difficult due to the diversity of invertebrates and their structural and physiological characteristics. Research involving invertebrate cell cultures continuous to increase, although the number of cell lines used in still limited. This manual gives detailed descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro. Nutritional requirements, cultures media and species -specific methods for both cell and organ cultures as well as useful techniques for studies on cultured cells are described .The appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions. This comprehensive manual, the first of its kind, is a valuable reference for investigator working with invertebrate cell cultures in academia and industry. ISBN:9788184898699 178 Yr. of Pub.2011 Paper Back English
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